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E-MTAB-5800

ABSTRACT: RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96

PROVIDER: E-MTAB-5800 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-5800-analysis-methods.tsv	Tabular
	E-MTAB-5800-analytics.tsv	Tabular
	E-MTAB-5800-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5800-configuration.xml	Xml
	E-MTAB-5800-g2_g1-mvaPlot.png	Other

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1 - 5 of 27

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RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96

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RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96

Project description:The microRNA miR-96 is important for hearing; mutations in the seed region result in dominant progressive hearing loss in mice and humans. Mir96 is expressed in the sensory hair cells of the organ of Corti along with Mir182 and Mir183. miR-96 is a master regulator of hair cell development, controlling many genes in the organ of Corti, but the role of miR-182 and miR-183 in the hair cells is unknown. We carried out RNA-seq on mice carrying a knockout allele of Mir182, and mice carrying a double knockout allele of Mir183 and Mir96 (Mir183/96). RNA was extracted from the organ of Corti from P4 homozygotes and sex-matched wildtype littermates. Strand-specific libraries were prepared using the NuGEN Ovation Mouse RNA-Seq System 1-16 kit and sequenced on an Illumina HiSeq 2500 machine as paired-end 125bp reads.

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