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E-MTAB-7520

ABSTRACT: RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

PROVIDER: E-MTAB-7520 | ExpressionAtlas | 2019-01-16

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-7520-analysis-methods.tsv	Tabular
	E-MTAB-7520-analytics.tsv	Tabular
	E-MTAB-7520-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7520-configuration.xml	Xml
	E-MTAB-7520-g3_g1-mvaPlot.png	Other

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RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

Project description:RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

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Project description:This experiment was designed to investigate how inhibition of Shh signalling result in loss of flight feathers, and to identify genes involved in flight feather development. We carried out cyclopamine treatment of wing buds at HH19 which gives rise to 2 digit wings with absence of flight feathers. The posterior forewing regions of control and cyclopamine treated wings were analysed at E10.5, the developmental stage at which flight feathers first become apparent. Control leg tissue was also included in the analysis to enrich data for genes involved in general feather development. To enrich further for genes with roles in flight feather development, we also included samples from the feathered legs of Pekin bantam chickens. 3 biological replicates of pooled wing/leg sections were analysed and 12 samples were sequenced.

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