Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MTAB-8860

ABSTRACT: RNA-seq of the human HCC cell line PLC5 after 72 hours treatment with either MAGEA3 shRNA or Scramble control

PROVIDER: E-MTAB-8860 | ExpressionAtlas | 2025-01-16

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of the human HCC cell line PLC5 after 72 hours treatment with either MAGEA3 shRNA or Scramble control

Project description:RNA-seq of the human HCC cell line PLC5 after 72 hours treatment with either MAGEA3 shRNA or Scramble control

| PRJEB37144 | ENA

RNA-seq of the human HCC cell line PLC5 after 72 hours treatment with either MAGEA3 shRNA or Scramble control

Project description:To better understand the mechanism by which MAGEA3 contributes to HCC progression, we conducted RNA sequencing in the PLC5 HCC cell line after 72 hours of treatment with scramble or sh8375 in triplicate. RNA-sequencing was conducted on poly-A enriched RNA, 100 bp single reads using an Illumina HiSeq2500 instrument. Libraries were constructed using the TruSeq RNA Library Prep Kit v2. Raw sequencing reads were mapped to the GRCh38 reference genome (USCS) using STAR (2.4.2g1). Aligned reads were mapped to GRCh38 genetic features using featureCounts from the subRead package with default settings. Data analysis included differential gene expression between conditions and gene set enrichment analysis.

2021-05-19 | E-MTAB-8860 | biostudies-arrayexpress

Development of gene expression signatures of CTCF-expressing vs CTCF-depleted hepatocellular carcinoma cells

Project description:To understand the functional role of CTCF in the oncogenesis of HCC, we constructed lentiviruses expressing control shRNA (shCont) or shRNA agains CTCF (shCTCF) and transduced them into PLC5 cells respectively. After 24 hours, cells were cultered in puromycin containing medium (5 ug) for another 24 hours. Cells were returned to normal medium for another 48 hours before total mRNAs were corrected for whole genome gene expression array analysis. Expression of FoxM1 gene from this signature was quantified in an independent set of RNA samples by real-time PCR, confirming differntial expression of this gene in shCTCF expressing cells.

2017-09-30 | GSE100533 | GEO

E-MTAB-5285

Project description:RNA-seq of A431 cell line after gefitinib treatment

2025-01-23 | E-MTAB-5285 | ExpressionAtlas

E-MTAB-6756

Project description:Total RNA-seq of human oesophageal adenocarcinoma cell line OE19 treated with siNT, siHNF4A, siGATA6 and siHNF4A+siGATA6 for 72 hours

2025-01-23 | E-MTAB-6756 | ExpressionAtlas

E-MTAB-7284

Project description:RNA-seq of human cancer cell line OVCA420* following TIGAR knockdown with shRNA

2025-01-23 | E-MTAB-7284 | ExpressionAtlas

E-MTAB-6244

Project description:RNA-seq of human U2OS cells depleted of NUAK1 by shRNA compared to control

2025-01-23 | E-MTAB-6244 | ExpressionAtlas

E-MEXP-2238

Project description:Transcription profiling of mouse embryonic stem cell line CGR8 transfected with control shRNA and shRNA against HP1g in two different developmental stages

2015-10-22 | E-MEXP-2238 | ExpressionAtlas

E-MTAB-8446

Project description:RNA-seq of OE19 cells treated with siNT or siKLF5 for 72 hours

2025-01-16 | E-MTAB-8446 | ExpressionAtlas

E-MTAB-8579

Project description:RNA-seq of OE19 cells treated with siNT or siERBB2 for 72 hours

2025-01-16 | E-MTAB-8579 | ExpressionAtlas