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E-TABM-142

ABSTRACT: Transcription profiling of wild type and loss-of-function mutants of Mla1 and Mla6 powdery mildew resistant allele barley plants

OTHER RELATED OMICS DATASETS IN: PRJNA261734PRJNA149125

PROVIDER: E-TABM-142 | ExpressionAtlas | 2015-12-04

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-TABM-142-analysis-methods.tsv	Tabular
	E-TABM-142-atlasExperimentSummary.Rdata	Rdata
	E-TABM-142-configuration.xml	Xml
	E-TABM-142-configuration.xml.beforeQC	Xml
	E-TABM-142-percentile-ranks.tsv	Tabular

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Transcription profiling of wild type and loss-of-function mutants of Mla1 and Mla6 powdery mildew resistant allele barley plants

Project description:A parallel expression profiling of wild-type and loss-of-function mutants of Mla6 and Mla1 powdery mildew resistance alleles was conducted using Barley1 GeneChip. Barley plants were inoculated with powdery mildew isolate 5874 and first leaves were harvested at 6 time points after pathogen inoculation. This experiment was conducted in split-split-plot experimental design with 3 replications.

2008-11-19 | E-TABM-142 | biostudies-arrayexpress

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Transcription profiling of Arabidopsis wild type and pen3 mutant plants inoculated with powdery mildew organisms Erysiphe cichoracearum or Blumeria graminis hordei

Project description:Arabidopsis is a host to the fungal powdery mildew pathogen, Erysiphe cichoracearum, and a nonhost to Blumeria graminis f.sp. hordei, the powdery mildew pathogenic on barley. A screen for mutants that allowed increased entry by this inappropriate or nonhost pathogen on Arabidopsis led to the identification of PEN3. While pen3 mutants permitted both increased penetration and increased hyphal growth by B. g. hordei, they were unexpectedly resistant to E. cichoracearum. This resistance was correlated with the appearance of chlorotic patches and was salicylic acid-dependent. Consistent with this observation, microarray analysis revealed that the salicylic acid defense pathway was hyper-induced in pen3 relative to wild type following inoculation with either E. cichoracearum or B. g. hordei. The pen3 phenotypes result from a loss of function of AtPDR8, a ubiquitously and highly expressed ATP binding cassette transporter. PEN3 protein tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated to high levels at infection sites and surrounded fungal penetration pegs. We hypothesize that PEN3 may be involved in exporting toxic substrates to sites of infection and that accumulation of these substrates intracellularly in the pen3 mutant may secondarily activate the salicylic acid pathway. Experiment Overall Design: Three week-old wild-type Col and mutant pen3 Arabidopsis thaliana plants were inoculated with Erysiphe cichoracearum, Blumeria graminis hordei, or not inoculated. 1 day post inoculation 16 rosettes were harvested per replicate. 4 replicates were perfomerd per treatment.

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