CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes

ABSTRACT: Proper chromosome segregation is essential to avoid aneuploidy, yet in mammalian oocytes it progressively fails in an age-dependent manner. Proteins contributing to correct chromosome segregation are therefore of central interest. In somatic cells, the scarcely described protein CENP- V was shown to contribute to chromosome structure and segregation, and to microtubule organisation. Here we report a key role for CENP-V in oocyte spindle formation and chromosome segregation. Depending on the maturation state of oocytes CENP-V localizes to and near the centromeres, to MTOCs, and to spindle microtubules. CENP-V deficient oocytes arrest at metaphase I, show strongly reduced polar body extrusion and increased numbers of mis-aligned metaphase I (MI) and metaphase II (MII) chromosomes. Multipola

ORGANISM(S): Mus musculus (mouse)

SUBMITTER: Dalileh Nabi 

PROVIDER: S-BSST699 | bioimages |

REPOSITORIES: bioimages