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Jeon2018 - Enzyme clustering in Glucose metabolism

ABSTRACT: Miji Jeon, Hye-Won Kang & Songon An. A Mathematical Model for Enzyme Clustering in Glucose Metabolism. Scientific Reports 8, 1 (2018). We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging. In this work, we develop a mathematical model using a system of ordinary differential equations, in which the association of the rate-limiting enzymes into multienzyme complexes is included as an essential element. We then demonstrate that our mathematical model provides a quantitative principle to simulate glucose flux at both subcellular and population levels in human cancer cells. Lastly, we use the model to simulate 2-deoxyglucose-mediated alteration of glucose flux in a population level based on subcellular high-content imaging data. Collectively, we introduce a new mathematical model for human glucose metabolism, which promotes our understanding of functional roles of differently sized multienzyme complexes in both single-cell and population levels.

SUBMITTER: Mikal Daou

PROVIDER: BIOMD0000001055 | BioModels | 2024-09-02

REPOSITORIES: BioModels

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			Action	DRS
	BIOMD0000001055?filename=Enzyme_clustering_in%20glucose_metabolism-biopax2.owl	Owl
	BIOMD0000001055?filename=Enzyme_clustering_in%20glucose_metabolism-biopax3.owl	Owl
	BIOMD0000001055?filename=Enzyme_clustering_in%20glucose_metabolism-matlab.m	Other
	BIOMD0000001055?filename=Enzyme_clustering_in%20glucose_metabolism-octave.m	Other
	BIOMD0000001055?filename=Enzyme_clustering_in%20glucose_metabolism.cps	Other

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Jeon2018 - Enzyme clustering in Glucose metabolism

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