Dataset Information

Haut1974_Pentose_Cycle_Rat

ABSTRACT: This is the Unlabelled model as described in: Simulation of the Pentose Cycle in Lactating Rat Mammary Gland Haut MJ , London JW , Garfinkel D Biochem J. 1974,138(3), 511-524. PMID: 4154746 ;PMCID: PMC1166237 Abstract: A computer model representing the pentose cycle, the tricarboxylic acid cycle and glycolysis in slices of lactating rat mammary glands has been constructed. This model is based primarily on the studies, with radioactive chemicals, of Abraham and Chaikoff (1959) [although some of the discrepant data of Katz and Wals (1972) could be accommodated by changing one enzyme activity]. Data obtained by using [1-(14)C]-, [6-(14)C]- and [3,4-(14)C]-glucose were simulated, as well as data obtained by using unlabelled glucose (for which some new experimental data are presented). Much past work on the pentose cycle has been mainly concerned with the division of glucose flow between the pentose cycle and glycolysis, and has relied on the assumption that the system is in steady state (both labelled and unlabelled). This assumption may not apply to lactating rat mammary glands, since the model shows that the percentage flow through the shunt progressively decreased for the first 2h of a 3h experiment, and we were unable to construct a completely steady-state model. The model allows examination of many quantitative features of the system, especially the amount of material passing through key enzymes, some of which appear to be regulated by NADP(+) concentrations as proposed by McLean (1960). Supplementary information for this paper has been deposited as Supplementary Publication SUP 50023 at the British Museum (Lending Division) (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973) 131, 5. All reactions are modelled as mass action, reactions labelled 1-30 in the order they appear in Scheme 1. Note that the unit of substance is micromole per 0.5g wet weight of tissue. Simulation reproduces results in tables 5 and 6. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. . To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

SUBMITTER: Kieran Smallbone

PROVIDER: MODEL1004070000 | BioModels | 2005-01-01

REPOSITORIES: BioModels

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			Action	DRS
	MODEL1004070000?filename=MODEL1004070000-biopax2.owl	Owl
	MODEL1004070000?filename=MODEL1004070000-biopax3.owl	Owl
	MODEL1004070000?filename=MODEL1004070000.m	Other
	MODEL1004070000?filename=MODEL1004070000.pdf	Pdf
	MODEL1004070000?filename=MODEL1004070000.png	Other

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Publications

Simulation of the pentose cycle in lactating rat mammary gland.

Haut M J MJ London J W JW Garfinkel D D

The Biochemical journal 19740301 3

A computer model representing the pentose cycle, the tricarboxylic acid cycle and glycolysis in slices of lactating rat mammary glands has been constructed. This model is based primarily on the studies, with radioactive chemicals, of Abraham & Chaikoff (1959) [although some of the discrepant data of Katz & Wals (1972) could be accommodated by changing one enzyme activity]. Data obtained by using [1-(14)C]-, [6-(14)C]- and [3,4-(14)C]-glucose were simulated, as well as data obtained by using unla ...[more]

PMID: 4154746

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