Dataset Information

Yeo2020 - Genome-scale model of CHO cells

ABSTRACT: The iCHO2291 model was reconstructed by updating the previously published iCHO1766 model in a 6-step procedure: 1) identification and removal of duplicate metabolites and reactions, 2) replacement of lumped reactions into detailed steps and removal of biochemically inconsistent reactions, 3) update of GPR based on latest genome annotations (as on Nov 1, 2018), 4) correction of GPR and reaction compartment assignment based on subcellular localization, 5) inclusion of new reactions based on new genome annotations, and 6) metabolic gap identification and resolution.

SUBMITTER: Meiyappan Lakshmanan

PROVIDER: MODEL1912180001 | BioModels | 2020-05-28

REPOSITORIES: BioModels

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Publications

Enzyme capacity-based genome scale modelling of CHO cells.

Yeo Hock Chuan HC Hong Jongkwang J Lakshmanan Meiyappan M Lee Dong-Yup DY

Metabolic engineering 20200421

Chinese hamster ovary (CHO) cells are most prevalently used for producing recombinant therapeutics in biomanufacturing. Recently, more rational and systems approaches have been increasingly exploited to identify key metabolic bottlenecks and engineering targets for cell line engineering and process development based on the CHO genome-scale metabolic model which mechanistically characterizes cell culture behaviours. However, it is still challenging to quantify plausible intracellular fluxes and d ...[more]

PMID: 32330653

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