Effect of in vitro transcription time on the fidelity of T7-based RNA linear amplification
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Hongjuan Zhao
PROVIDER: E-GEOD-3558 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis.
Zhao Hongjuan H Hastie Trevor T Whitfield Michael L ML Børresen-Dale Anne-Lise AL Jeffrey Stefanie S SS
BMC genomics 20021030 1
<h4>Background</h4>T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinomas and high-density cDNA microarrays.<h4>Results</h4>Using an optimized protocol, the average correlation coefficient of gene expression of 11,123 cDNA clones between amplified and unamplified ...[more]
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