Dataset Information

Suthers2020 - Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 - iIsor850

ABSTRACT: Published 2020 version of the first genome-scale metabolic model for Issatchenkia orientalis SD108. Abstract: Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial production of organic acids by microorganisms. Issatchenkia orientalis SD108 is a promising host for industrial production because it is tolerant to acidic conditions as low as pH 2.0. With the goal to systematically assess the metabolic capabilities of this non-model yeast, we developed a genome-scale metabolic model for I. orientalis SD108 spanning 850 genes, 1826 reactions, and 1702 metabolites. In order to improve the model's quantitative predictions, organism-specific macromolecular composition and ATP maintenance requirements were determined experimentally and implemented. We examined its network topology, including essential genes and flux coupling analysis and drew comparisons with the Yeast 8.3 model for Saccharomyces cerevisiae. We explored the carbon substrate utilization and examined the organism's production potential for the industrially-relevant succinic acid, making use of the OptKnock framework to identify gene knockouts which couple production of the targeted chemical to biomass production. The genome-scale metabolic model iIsor850 is a data-supported curated model which can inform genetic interventions for overproduction.

SUBMITTER: Patrick F Suthers

PROVIDER: MODEL2204190004 | BioModels | 2023-11-27

REPOSITORIES: BioModels

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			Action	DRS
	MODEL2204190004?filename=FROG.omex	Other
	MODEL2204190004?filename=MemoteReport.html	Other
	MODEL2204190004?filename=Suthers2020_iIsor850.xml	Xml
	MODEL2204190004?filename=iIsor850%20miniFROG.xlsx	Xlsx

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Publications

Genome-scale metabolic reconstruction of the non-model yeast <i>Issatchenkia orientalis</i> SD108 and its application to organic acids production.

Suthers Patrick F PF Dinh Hoang V HV Fatma Zia Z Shen Yihui Y Chan Siu Hung Joshua SHJ Rabinowitz Joshua D JD Zhao Huimin H Maranas Costas D CD

Metabolic engineering communications 20201008

Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial production of organic acids by microorganisms. <i>Issatchenkia orientalis</i> SD108 is a promising host for industrial production because it is tolerant to acidic conditions as low as pH 2.0. With the goal to systematically assess the metabolic capabilities of this no ...[more]

PMID: 33134082

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