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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Yersinia pestis dam mutant that has attenuated virulence in mice

ABSTRACT: We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-32

ORGANISM(S): Yersinia pestis

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PROVIDER: E-BUGS-32 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A dam mutant of Yersinia pestis is attenuated and induces protection against plague.

Robinson Victoria L VL Oyston Petra C F PC Titball Richard W RW

FEMS microbiology letters 20050915 2

We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results ...[more]

PMID: 16188402

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