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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Foxa2 knock-outs in mice to better define the role of this gene in glucose homeostasis

ABSTRACT: Study to further characterize the genetic and functional consequence of the beta cell-specific ablation of Foxa2 (hepatocyte nuclear factor 3 beta, HNF-3b) in mice to better define the role of this gene in glucose homeostasis. The study involved 8 two-channel assays on UPenn Mouse PancChip 4.0, each consisting of a competitive mutant vs control hybridization. 4 control and 4 Foxa2loxP/loxP;Ins.CRE RNA samples were hybridized to the array using a dye-swap design. (The results from one of these assays had to be discarded from the analyses, so 7 assays were used.)

ORGANISM(S): Mus musculus

SUBMITTER: John Brestelli

PROVIDER: E-CBIL-13 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Foxa2 regulates multiple pathways of insulin secretion.

Lantz Kristen A KA Vatamaniuk Marko Z MZ Brestelli John E JE Friedman Joshua R JR Matschinsky Franz M FM Kaestner Klaus H KH

The Journal of clinical investigation 20040801 4

The regulation of insulin secretion by pancreatic beta cells is perturbed in several diseases, including adult-onset (type 2) diabetes and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). The first mouse model for PHHI has a conditional deletion of the gene encoding the winged-helix transcription factor Foxa2 (Forkhead box a2, formerly Hepatocyte nuclear factor 3beta) in pancreatic beta cells. Using isolated islets, we found that Foxa2 deficiency resulted in excessive insulin release ...[more]

PMID: 15314688

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