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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse islets treated with PANcreatic DERived factor for 48 or 72 h to elucidate the genetic mechanisms of PANDER-induced cell death

ABSTRACT: Expression profiling using the UPenn Mouse PancChip 5.0 was used to elucidate the genetic mechanisms of PANDER-induced cell death in pancreatic islets. Murine islets were treated with PANDER (PANcreatic DERived factor) for 48 or 72 h (n=4 and n=3 respectively). Following linear amplification, the RNA was matched for purity using Quantitative PCR.

ORGANISM(S): Mus musculus

SUBMITTER: Bryan Wolf

PROVIDER: E-CBIL-7 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21).

Burkhardt Brant R BR Greene Scott R SR White Peter P Wong Ryan K RK Brestelli John E JE Yang Jichun J Robert Claudia E CE Brusko Todd M TM Wasserfall Clive H CH Wu Jianmei J Atkinson Mark A MA Gao Zhiyong Z Kaestner Klaus H KH Wolf Bryan A BA

Gene 20060118

PANcreatic DERived factor is an islet-specific cytokine that promotes apoptosis in primary islets and islet cell lines. To elucidate the genetic mechanisms of PANDER-induced cell death we performed expression profiling using the mouse PancChip version 5.0 in conjunction with Ingenuity Pathway Analysis. Murine islets were treated with PANDER and differentially expressed genes were identified at 48 and 72 h post-treatment. 64 genes were differentially expressed in response to PANDER treatment. 22 ...[more]

PMID: 16412588

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