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Transcription profiling of mouse skeletal muscle in streptozotocin diabetes with or without insulin treatment

ABSTRACT: Metabolic abnormalities underlying diabetes are primarily the result of the lack of adequate insulin action and the associated changes in protein phosphorylation and gene expression. Affymetrix oligonucleotide microarrays were used to study the changes in the transcriptional program of mouse skeletal muscle in insulin-deficient diabetes. Mice which were made diabetic by streptozotocin treatment were compared to controls. Also, the reversibility of these changes was ascertained by treating a subset of the diabetic mice with insulin.

INSTRUMENT(S): Affymetrix Fluidics Station 400

ORGANISM(S): Mus musculus

SUBMITTER: Ronald Kahn

PROVIDER: E-CBIL-25 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Coordinated patterns of gene expression for substrate and energy metabolism in skeletal muscle of diabetic mice.

Yechoor Vijay K VK Patti Mary-Elizabeth ME Saccone Robert R Kahn C Ronald CR

Proceedings of the National Academy of Sciences of the United States of America 20020729 16

Metabolic abnormalities underlying diabetes are primarily the result of the lack of adequate insulin action and the associated changes in protein phosphorylation and gene expression. To define the full set of alterations in gene expression in skeletal muscle caused by diabetes and the loss of insulin action, we have used Affymetrix oligonucleotide microarrays and streptozotocin-diabetic mice. Of the genes studied, 235 were identified as changed in diabetes, with 129 genes up-regulated and 106 do ...[more]

PMID: 12149437

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