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Transcription profiling of mouse neuron-specific mRNA complexity responses during hippocampal apoptosis following traumatic brain injury

ABSTRACT: Study aimed at examining differential expression between single apoptotic neurons from both the CA-3 and dentate and those in an un-injured brain.

ORGANISM(S): Mus musculus

SUBMITTER: Tracy McIntosh

PROVIDER: E-CBIL-4 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury.

Marciano Paolo G PG Brettschneider Julia J Manduchi Elisabetta E Davis Jason E JE Eastman Scott S Raghupathi Ramesh R Saatman Kathryn E KE Speed Terence P TP Stoeckert Christian J CJ Eberwine James H JH McIntosh Tracy K TK

The Journal of neuroscience : the official journal of the Society for Neuroscience 20040301 12

In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compar ...[more]

PMID: 15044525

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