Response of myeloid lineage cells to cytokines
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ABSTRACT: This study is focused to find new genes involved in Insulin signalling in bone marrow derived macrophages upon treatment with IL-4 (10 ng/ml) and LPS (100 nM). We will use primary macrophages differentiated from bone-marrow derived hematopoetic stem cells; our aim is to analyze the transcriptome upon anti-inflammatory (IL-4) and inflammatory (LPS) treatment.Total RNA was extracted with RNAEasy kit form Qiagen using manufacturer reccomendations and RIN values for all samples were between 9 and 10.
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Mus musculus
SUBMITTER:
PROVIDER: E-ERAD-165 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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