Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Single cell gene expression patterns across different cell cycle stages in mouse embryonic cells

ABSTRACT: This study is aimed to decipher the gene expression patterns that exist across the different cell cycle stages at level of individual cells and their inherent heterogeneity. Conventional studies in yeast and human cells have shown coordination between gene expression and cell-cycle. However such a study for mouse ES cells (mES) and their differentiation has not been performed at a single cell level. In this study, we obtain pure mES cells from different cell cycle stages using FACS and apply single cell RNA-sequencing using the Fluidigm C1 system. This work will decipher and unravel the transcriptional patterns that coordinated with cell cycle progression at a single cell level.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-ERAD-240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:The goal of this project is to understand the etiology and pathology of host-pathogen interaction using high-throughput cellular phenotyping. By combining the expertise in pathogen biology, informatics and stem cell biology across the institute, this project will use targeted genetic modification in mouse embryonic stem cells to identify functions of host genes in innate immunity. We will also explore the biology of mouse embryonic stem cells and their differentiation into immune competent cells. To this end we will generate homozygous and inducible knockout lines in mouse embryonic stem cells, which will be differentiated into immune competent cells, such as macrophages or dendritic cells. These will be subjected to a panel of immune challenge and stimulation assays to characterise the functions of the disrupted genes. To identify new functions for mammalian genes in innate immunity we will:\Generate a panel of homozygous inducible gene knockout mouse ES cell lines working from a list of ca. 80 candidate genes (MGP phenotyping and GWAS hits). \Scale up differentiation protocols for immune challenge and stimulation assays.\Benchmark in vitro differentiated ES cell models against primary immune cells from selected MGP mouse lines and eventually against human iPS cells.\Screen a panel of 30 genetically modified cell lines in immunological challenge and stimulation assays, defining phenotypes at the cellular level by high content imaging, biochemically by assaying cytokine production, and at the molecular level by transcriptome analysis.\Define novel gene functions in innate immunity through network analysis of gene expression data from all challenged cell lines.\In the longer term scale up the production and phenotyping of genetically modified cell lines (mouse or human as appropriate).This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Dataset Information

Single cell gene expression patterns across different cell cycle stages in mouse embryonic cells

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets