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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA sequencing of mouse haemopoietic cells 2014 15

ABSTRACT: Comprehensive analysis of AML genomes has revealed co-occurring mutations in DNA andhistone modifying enzymes suggesting their crucial role in tumourigenesis. To gain inside intothe role of those genes in AML we generated mouse models recapitulating mutations in partof the most frequent ones. We hypothesise that aberrant chromatin modification andunderlying effect on gene expression largely contribute to leukemogenesis. To test thishypothesis we propose to compare gene expression and DNA and histone modification in thepresence or absence of single gene mutation in mouse hematopoietic stem and progenitorcompartment in a pre-leukaemia setting. Characterisation of these models will inform thebiology of AML, identify potential therapeutic targets and provide platforms for theassessment of novel therapeutics.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-ERAD-322 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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