Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

29 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dynamic Equilibrium of human ESC populations

ABSTRACT: ES cells grown in culture are heterogeneous with respect to their transcriptional profiles, their methylation landscape and, functionally, their potency. This study is investigating overall changes in transcriptional landscape of ES cells upon overexpression of epigenetic regulators which can indicate a shift in the distribution of subpopulations.Epigenetic regulators are expressed in human embryonic stem cells, and their effect on the transcriptional landscape is analysed.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER:

PROVIDER: E-ERAD-329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Study of gene expression in mouse ESC early differentiation

Project description:Mouse Rex1GFPd2 ESCs were induced to differentiation, samples at early differentiation stages were colleced for RNA-Seq to study the gene expression changes during initiation of differentiation.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2015-11-03 | E-ERAD-403 | biostudies-arrayexpress

ESC Methylome Dynamics

Project description:Mouse Embryonic Stem Cells (ESCs) are grown in culture and seperated based upon heterogeneous expression of key pluripotency factors. DNA was obtained from cells with the Qiagen AllPrep kit. 0.2ug of DNA was sonicated with the Covaris Sonicator (300bp) and the Illumina library was prepared following a standard Illumina protocol with the NEB Next kit. The library was amplified 15x and size-selected within the range of 200-500bp. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-08-07 | E-ERAD-178 | biostudies-arrayexpress

RNA Seq of P berghei ANKA life cycle stages

Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including important human pathogens. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages of the mouse malaria parasite, P. berghei ANKA. The aim is to make transcriptional landscape maps of different life cycle stages of P. berghei ANKA at single base pairs resolution. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-02-21 | E-ERAD-250 | biostudies-arrayexpress

Epigenetic regulation of pre implantation development

Project description:Pre-implantation development is exemplified by extensive transcriptional and epigenetic changes, including zygotic genome activation, chromatin remodelling and global DNA demethylation. Excitingly, the advent of single-cell technologies will enable unprecedented high-resolution transcriptional and epigenetic profiling of this critical developmental stage. Furthermore, through the development of an in vitro model system I will identify key totipotency regulators, develop mechanistic insight into their mode of action and link these findings to somatic reprogramming. This study involves the use of RNA-sequencing (polyA+, ribominus and single-cell), ChIP-Sequencing and bisulfite sequencing.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2015-06-30 | E-ERAD-370 | biostudies-arrayexpress

Transcriptomics study of mouse and human macrophage and intestinal organoids in response to pathogen

Project description:Aim of this study is to understand the transcriptional response of macrophages and intestinal epithelial cells to pathogen challenge.These data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-11-18 | E-ERAD-310 | biostudies-arrayexpress

Survival of Salmonella enterica serotype Typhi in the water microcosm transcriptome response

Project description:Investigation of the transcriptional response of S. Typhi strain BRD948 during adaptation to the watery microcosm using RNA sequencing. http://www.sanger.ac.uk/resources/downloads/bacteria/salmonella.html This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-01-07 | E-ERAD-205 | biostudies-arrayexpress

Tel AML1 mtumours

Project description:This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/In this experiment we sequenced the transcriptomes of mouse Tel-AML1 tumours to identify transcriptional profiles associated with the development of B-ALL.

2014-08-12 | E-ERAD-264 | biostudies-arrayexpress

ZF adult transcriptome

Project description:Total RNA was extracted from adult male and female zebrafish. Illumina cDNA libraries were prepared from polyA+ RNA and sequenced using Illumina HiSeq paired-end sequencing. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ protocol: Total RNA was extracted from zebrafish embryos using Trizol and DNase treated. Libraries were made using the Illumina cDNA protocol from polyA+ RNA.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-01-07 | E-ERAD-54 | biostudies-arrayexpress

ZFP106 expression profile

2013-03-11 | E-ERAD-111 | biostudies-arrayexpress

ChIP Seq Based Gene Screening in Mice

2014-01-28 | E-ERAD-187 | biostudies-arrayexpress