Identification of Mysm1 genomic binding sites by ChIP Seq
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ABSTRACT: We performed chromatin immunoprecipitation according to standard protocols. Mouse embryonic stem cells were treated with formaldehyde to cross-link proteins to DNA. Cell lysates were sonicated to break up DNA. The histone deubiquitinase Mysm1 was then immunoprecipitated from the sonicated cell lysates. Mysm1-bound (and control antibody-bound) DNA fragments were purified after proteinase K treatment and cross-link reversal. Input DNA was purified directly from the sonicated cell lysates. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Mus Musculus
SUBMITTER:
PROVIDER: E-ERAD-398 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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