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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Developmental and molecular heterogeneity of mouse hematopoietic stem cells

ABSTRACT: This study aims to investigate the developmental and molecular heterogeneity of mouse hematopoietic stem cell (mHSC). The genome-wide transcriptomic heterogeneity in wildtype and Bcl11a deficient mHSC will be stuided by single-cell mRNA sequencing technology. Brief protocol:Adult mouse LSK HSC is harvested from femur and FACS purified by surface markers CD48-, CD150+. Single cell capture and cDNA preparation are performed by Fluidigm C1 microfluidic system according to manufacturer protocol. Library is prepared by Illumina Nextera XT kit.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

DISEASE(S): WT

SUBMITTER:

PROVIDER: E-ERAD-407 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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