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Single cell RNA-seq of 444 epithelial cells from the mammary glands of pubescent and adult mice

ABSTRACT: Mammary glands were collected from 8 pubescent (4.7-4.9 week-old) female mice and 8 adult (10 week old) female mice. Freshly sorted epithelial cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. Cells were visualized under the microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were prepared using the Nextera XT kit and sequencing was carried out on an Illumina NextSeq 500 to achieve paired-end 75 bp reads.

ORGANISM(S): Mus musculus

PROVIDER: GSE95445 | GEO | 2017/11/20

SECONDARY ACCESSION(S): PRJNA377129

REPOSITORIES: GEO

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