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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA seq on olfactory epithelium cryosections

ABSTRACT: Olfactory sensory neurons (OSNs) expressing the same olfactory receptor (OR) are randomlydispersed within the same olfactory epithelial zone. To date, between 4 and 12 zones havebeen defined based on OR expression by in-situ hybridization studies. However, the totalnumber of zones, their organisation, molecular identity, and their distribution across thedorsal-ventral, anterior-posterior and lateral-medial axes are still unknown. To address thesequestions we propose to perform RNA-seq on sequential cryosections along the dorsalventraland anterior-posterior axes. This will provide a spatial dimension to the quantitativedata we already have on the number of each neuron type in the entire epithelium. We aim touse this as preliminary data for a grant application to completely resolve the 3D organisationof the olfactory epithelium at a cellular level. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

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PROVIDER: E-ERAD-433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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