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Circadian regulation of a limited set of microRNAs in Drosophila

ABSTRACT: We used a microarray platform to survey the daily levels of 78 D. melanogaster miRNAs in adult heads of wildtype control flies and the arrhythmic clock mutant cyc01. At least 2 miRNAs were identified that showed robust daily abundance changes in control flies but not the cyc01 mutant. Keywords: microRNA, Circadian regulation yw and yw;cyc01 flies were maintained in standard media. For collections, approximately 50-100 young (2-5 day old) flies were placed in vials that were incubated at 25oC for four days in standard 12hr light-12hr dark cycles [LD; where zeitgeber time 0 (ZT0) is defined as lights-on]. Flies were collected by freezing in dry ice during the fourth LD cycle at the following times; ZT1, 7, 13 and 19. Subsequently low molecular RNAs were extracted for microarray analysis using a mirMAX microarray.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Maocheng Yang

PROVIDER: E-GEOD-10005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Circadian regulation of a limited set of conserved microRNAs in Drosophila.

Yang Maocheng M Lee Jung-Eun JE Padgett Richard W RW Edery Isaac I

BMC genomics 20080219

<h4>Background</h4>MicroRNAs (miRNAs) are short non-coding RNA molecules that target mRNAs to control gene expression by attenuating the translational efficiency and stability of transcripts. They are found in a wide variety of organisms, from plants to insects and humans. Here, we use Drosophila to investigate the possibility that circadian clocks regulate the expression of miRNAs.<h4>Results</h4>We used a microarray platform to survey the daily levels of D. melanogaster miRNAs in adult heads o ...[more]

PMID: 18284684

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