Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Systematic evaluation of variability in simulated ChIP-chip experiments


ABSTRACT: The most widely used method for detecting genome-wide protein-DNA interactions is chromatin immunoprecipitation on tiling microarrays, commonly known as ChIP-chip. Here, we conducted the first objective analysis of tiling array platforms, amplification procedures, and signal detection algorithms in a simulated ChIP-chip experiment. Mixtures of human genomic DNA and "spike-ins" comprised of nearly 100 human sequences at various concentrations were hybridized to four tiling array platforms by eight independent groups. Blind to the number of spike-ins, their locations, and the range of concentrations, each group made predictions of the spike-in locations. We found that microarray platform choice is not the primary determinant of overall performance. In fact, variation in performance between labs, protocols and algorithms within the same array platform was greater than the variation in performance between array platforms. However, each array platform had unique performance characteristics that varied with tiling resolution and the number of replicates, which have implications for cost versus detection power. Long oligonucleotide arrays were slightly more sensitive at detecting very low enrichment. On all platforms, simple sequence repeats and genome redundancy tended to result in false positives. LM-PCR and WGA, the most popular sample amplification techniques, reproduced relative enrichment levels with high fidelity. Performance among signal detection algorithms was heavily dependent on array platform. The spike-in DNA samples and the data presented here provide a stable benchmark against which future ChIP platforms, protocol improvements, and analysis methods can be evaluated. Keywords: ChIP-chip See manuscript for details

ORGANISM(S): Homo sapiens

SUBMITTER: Bing Ren 

PROVIDER: E-GEOD-10112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Systematic evaluation of variability in ChIP-chip experiments using predefined DNA targets.

Johnson David S DS   Li Wei W   Gordon D Benjamin DB   Bhattacharjee Arindam A   Curry Bo B   Ghosh Jayati J   Brizuela Leonardo L   Carroll Jason S JS   Brown Myles M   Flicek Paul P   Koch Christoph M CM   Dunham Ian I   Bieda Mark M   Xu Xiaoqin X   Farnham Peggy J PJ   Kapranov Philipp P   Nix David A DA   Gingeras Thomas R TR   Zhang Xinmin X   Holster Heather H   Jiang Nan N   Green Roland D RD   Song Jun S JS   McCuine Scott A SA   Anton Elizabeth E   Nguyen Loan L   Trinklein Nathan D ND   Ye Zhen Z   Ching Keith K   Hawkins David D   Ren Bing B   Scacheri Peter C PC   Rozowsky Joel J   Karpikov Alexander A   Euskirchen Ghia G   Weissman Sherman S   Gerstein Mark M   Snyder Michael M   Yang Annie A   Moqtaderi Zarmik Z   Hirsch Heather H   Shulha Hennady P HP   Fu Yutao Y   Weng Zhiping Z   Struhl Kevin K   Myers Richard M RM   Lieb Jason D JD   Liu X Shirley XS  

Genome research 20080207 3


The most widely used method for detecting genome-wide protein-DNA interactions is chromatin immunoprecipitation on tiling microarrays, commonly known as ChIP-chip. Here, we conducted the first objective analysis of tiling array platforms, amplification procedures, and signal detection algorithms in a simulated ChIP-chip experiment. Mixtures of human genomic DNA and "spike-ins" comprised of nearly 100 human sequences at various concentrations were hybridized to four tiling array platforms by eigh  ...[more]

Similar Datasets

2008-01-09 | GSE10090 | GEO
2008-01-09 | GSE10076 | GEO
2008-12-31 | GSE10112 | GEO
2007-12-13 | GSE9848 | GEO
2007-12-13 | GSE9849 | GEO
2008-02-01 | GSE10114 | GEO
2010-09-30 | E-GEOD-10114 | biostudies-arrayexpress
2007-12-12 | E-GEOD-9849 | biostudies-arrayexpress
2007-12-12 | E-GEOD-9848 | biostudies-arrayexpress
2010-05-26 | E-GEOD-10090 | biostudies-arrayexpress