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A Global Genomic View of MIF Knockdown Cell

ABSTRACT: siRNA-mediated knockdown of MIF expression in HEK293 cells resulted in inhibition of cell proliferation and cell cycle progress. The microarray study of MIF KD cells reveald knockdown of MIF would lead to extensive changes in gene expression profiles which may elucidate the molecular mechanism of MIF siRNA-mediated inhibition of cell cycle and cell proliferation. Keywords: The whole-genome expression analysis in MIF knockdown cells and the control cells HEK293 cells were transfected MIF siRNA (50pmol/ml & 100pmol/ml) or control RNA using LipofectamineTM 2000 reagent and re-incubated for 24 hours.At the designed time point, totol RNA was isolated from the different treated cells.The normal cells were used as control.

ORGANISM(S): Homo sapiens

SUBMITTER: Lingfeng Liu 

PROVIDER: E-GEOD-10241 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A global genomic view of MIF knockdown-mediated cell cycle arrest.

Liu Lingfeng L   Ji Chaoneng C   Chen Jinzhong J   Li Yao Y   Fu Xuping X   Xie Yi Y   Gu Shaohua S   Mao Yumin Y  

Cell cycle (Georgetown, Tex.) 20080324 11

Macrophage immigration inhibitory factor (MIF) is a ubiquitously expressed proinflammatory mediator that has also been implicated in cell growth, cell cycle and carcinogenesis. In this study, we demonstrate siRNA-mediated knockdown of MIF results in G(0)/G(1) cell cycle arrest in HEK293 cells. To elucidate the molecular mechanism of cell cycle perturbation following MIF knockdown, we employed microarray to investigate the genome-wide expression profile in MIF-deficient cells and normal cells. Qu  ...[more]

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