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Transcription profiling of mouse NKDxIL15tg and IL15 tg NK cells isolated from spleen and purified by FACS

ABSTRACT: NK cells from NKDxIL15tg mice spleens and bone marrow were purified by FACS. NK cells from IL15tg mice spleens were purified by FACS. Gene expression differences were analyzed to identify genes involved in NK cell development. Experiment Overall Design: Immature NK cells from NKDxIL15tg mice and mature NK cells from IL15tg mice were purified by FACS. RNA was made for target synthesis and hybridization to Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: John Sunwoo

PROVIDER: E-GEOD-10365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distal-less homeobox transcription factors regulate development and maturation of natural killer cells.

Sunwoo John B JB Kim Sungjin S Yang Liping L Naik Tina T Higuchi Darryl A DA Rubenstein John L JL Yokoyama Wayne M WM

Proceedings of the National Academy of Sciences of the United States of America 20080729 31

Natural killer (NK) cells constitute a subpopulation of lymphocytes that develop from precursors in the bone marrow (BM), but the transcriptional regulation of their development and maturation is only beginning to be understood, in part due to their relatively rare abundance, especially of developmental subsets. Using a mouse model in which NK cells are arrested at an immature stage of development, and a gene expression profiling approach, we uncovered transient normal NK cell expression of a ho ...[more]

PMID: 18664585

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