Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of nouse embryonic stem cells differentiated into embryoid bodies under normal growth conditions

ABSTRACT: Embryonic stem cells differentiated into embryoid bodies. Their expression pattern will be compared to that of the same cells grown in regular growth conditions. A study of the differential gene expression of these two populations should reveal genes whose expression is necessary for the maintenance of the stem cell phenotype. Experiment Overall Design: this experiment include 2 samples and 12 replicates

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-10404 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Embryonic stem cells differentiated into embryoid bodies. vs cells grown in regular growth conditions

Project description:Embryonic stem cells differentiated into embryoid bodies. Their expression pattern will be compared to that of the same cells grown in regular growth conditions. A study of the differential gene expression of these two populations should reveal genes whose expression is necessary for the maintenance of the stem cell phenotype. Keywords: other

2008-03-01 | GSE10404 | GEO

Expression data from Mst knockout embryoid bodies and wild type embryoid bodies

Project description:Mst1/Mst2 are central components of Hippo pathway. We examined the role of Mst1/Mst2 in ES cell differentiation. In this data set, we include expression data from day 4 and day 8 Mst1/Mst2 knockout ES cell formed embryoid bodies and wild type embryoid body controls.

2014-01-02 | GSE50219 | GEO

Geminin represses mesendoderm cell fate acquisition in embryoid bodies

2014-07-01 | GSE39676 | GEO

Expression data from Mst knockout embryoid bodies and wild type embryoid bodies

2014-01-02 | E-GEOD-50219 | biostudies-arrayexpress

Embryoid Bodies from Mouse R1 ES - 9 day

Project description:Embryoid Bodies from Mouse R1 ES - 9 day Keywords: other

2005-07-28 | GSE3007 | GEO

scRNA-seq of Homo sapiens differentiated primordial germ cell-like cell derived from embryoid bodies

Project description:We sequenced embryoid bodies at various time points following induction of pre-mesendoderm cells (PreME) towards primordial germ cell-like cell (PGCLC) fate

2023-05-05 | E-MTAB-11283 | biostudies-arrayexpress

11-Point Time Course Study of Differentiating J1 Embryoid Bodies

Project description:An 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days. Keywords: Time course

2005-12-06 | GSE3749 | GEO

Genome-wide maps of Rad21 in R1/E embryonic stem cells and R1/E derived Embryoid bodies

Project description:ChIP-seq based determination of Rad21 binding sites in embryonic stem cells (ESC) and ESC derived Embryoid Bodies (EB) identified

2011-05-19 | GSE24029 | GEO

Geminin represses mesendoderm cell fate acquisition in embryoid bodies

Project description:Geminin is a small nucleoprotein that neuralizes ectoderm in the Xenopus embryo. Geminin promotes neural fate acquisition of mouse embryonic stem cells: Geminin knockdown during neural fate acquisition decreased expression of neural precursor cell markers (Pax6, Sox1), while increasing expression of Pitx2, Lefty1 and Cited2, genes involved in formation of the mouse node. Here we differentiated mouse embryonic stem cells into embryoid bodies to study Geminin's ability to repress primitive streak mesendoderm fate acquisition. We used microarrays to define the sets of genes that are regulated by Geminin during cell fate acquisition in embryoid bodies, using Dox-inducible Geminin knockdown or overexpression mouse embryonic stem cell lines. ES cell lines for Geminin over-expression (GemOE) were treated without or with Dox from day 3 to day 5 of EB differentiation and were collected on days 4 or 5 for microarray analysis. Gem knockdown (KD) ES cell lines were treated without or with Dox from day 0 to day 4 of EB differentiation and were collected on day 4 for microarray analysis.

2014-07-01 | E-GEOD-39676 | biostudies-arrayexpress

Expression data from the inducible expression of EWS-FLI1 (EF) in embryoid bodies

Project description:Oncogenic transformation in Ewing sarcoma tumors is driven by the fusion oncogene EWS-FLI1. The inducible expression of EWS-FLI1 (EF) in embryoid bodies, or collections of differentiating stem cells, generates cells with properties of Ewing sarcoma tumors, including characteristics of transformation. These cell lines exhibit anchorage-independent growth, a lack of contact inhibition and a strong Ewing sarcoma gene expression signature. These cells also demonstrate a requirement for the persistent expression of EWS-FLI1 for cell survival and growth. We used microarrays to detail the effects of doxycycline-inducible expression of EWS-FLI1 on the gene expression signature of cells derived from differentiating stem cells, or embryoid bodies. Triplicate biological replicates were collected and analyzed.

2015-12-31 | E-GEOD-64686 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data