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Card9 dependent activation of macrophages by TDB

ABSTRACT: Bone marrow derived macrophages from wt and card9 KO mice were stimulated with CpG, Curdlan or TDB, an analogon to the mycobacterial cord factor TDM for 48h, respectively. Keywords: card9 knockout, macropphage, TDB wt or card9 KO macrophages stimulated for 48h

ORGANISM(S): Mus musculus

SUBMITTER: Joerg Mages

PROVIDER: E-GEOD-10530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adjuvanticity of a synthetic cord factor analogue for subunit Mycobacterium tuberculosis vaccination requires FcRgamma-Syk-Card9-dependent innate immune activation.

Werninghaus Kerstin K Babiak Anna A Gross Olaf O Hölscher Christoph C Dietrich Harald H Agger Else Marie EM Mages Jörg J Mocsai Attila A Schoenen Hanne H Finger Katrin K Nimmerjahn Falk F Brown Gordon D GD Kirschning Carsten C Heit Antje A Andersen Peter P Wagner Hermann H Ruland Jürgen J Lang Roland R

The Journal of experimental medicine 20090112 1

Novel vaccination strategies against Mycobacterium tuberculosis (MTB) are urgently needed. The use of recombinant MTB antigens as subunit vaccines is a promising approach, but requires adjuvants that activate antigen-presenting cells (APCs) for elicitation of protective immunity. The mycobacterial cord factor Trehalose-6,6-dimycolate (TDM) and its synthetic analogue Trehalose-6,6-dibehenate (TDB) are effective adjuvants in combination with MTB subunit vaccine candidates in mice. However, it is u ...[more]

PMID: 19139169

Similar Datasets

Card9 dependent activation of macrophages by TDB

Project description:Bone marrow derived macrophages from wt and card9 KO mice were stimulated with CpG, Curdlan or TDB, an analogon to the mycobacterial cord factor TDM for 48h, respectively. Keywords: card9 knockout, macropphage, TDB

2009-02-01 | GSE10530 | GEO

Transcription profiling of mouse bone marrow macrophages, comparison of CpG and TDB induced activation patterns

Project description:Bone marrow derived macrophages 1 ÂµM CpG or 20 Âµg/ml TDB, an analogon to the mycobacterial cord factor TDM for 8h, 24h, 48h and 72h respectively. Experiment Overall Design: time series; 8h, 24h, 48h and 72h CpG or TDB

2009-02-08 | E-GEOD-10532 | biostudies-arrayexpress

Comparison of CpG and TDB induced activation patterns in macrophages.

Project description:Bone marrow derived macrophages 1 µM CpG or 20 µg/ml TDB, an analogon to the mycobacterial cord factor TDM for 8h, 24h, 48h and 72h respectively. Keywords: CpG, TDB, macrophage, time series

2009-02-01 | GSE10532 | GEO

Mycobacterial cord factor reprograms the macrophage response to IFN-gamma towards enhanced inflammation yet impaired antigen presentation and expression of Gbp1

Project description:Mycobacteria can survive and persist in macrophages despite triggering multiple pattern recognition receptors and strong T cell-derived IFN-gamma production. The mycobacterial cord factor trehalose-6,6-dimycolate binds the C-type lectin receptor Mincle and induces inflammatory gene expression through the Syk-Card9 pathway. It is well established that TDM and IFN-gamma synergize to induce expression of iNOS. However, the cord factor may also act to down-regulate IFN-gamma-induced responses and contribute to mycobacterial immune evasion. Here, we have investigated the cross-regulation of the macrophage transcriptome by IFN-gamma and by TDM, as well as its synthetic analog TDB. For genome-wide expression profiling, bone marrow derived macrophages (BMMs) from female C57BL/6 mice aged 6 to 8 weeks were used for stimulation.

2019-09-10 | GSE137150 | GEO

Genome-wide analysis of the CARD9/Dectin-1 transcriptional program in neutrophils and macrophages by RNA-seq

Project description:Purpose: To characterize Card9-dependent and Card9-independent Dectin-1 mediated gene expression changes in neutrophils and to compare response to Dectin-1 stimulation in neutrophils and macrophages. Method: BM neutrophils from WT and Card9-/- mice were isolated by MACS column purification and treated with or without the Dectin-1 agonist, curdlan for 3 hrs. WT BM-derived macrophages were also treated with or without curdlan for 3hrs before sample collection. Results: We identified both Card9-dependent and Card9-independent gene expression changes with Dectin-1 stimulation in neutrophils. We also identified cell-type specific differences in the response to Dectin-1 stimulation in neutrophils and macrophages. Conclusions: Dectin-1 stimulation induces significant gene expression changes regulated by either canonical Card9-dependent or non-canonical Card9-independent mechanisms.

2021-02-26 | GSE148850 | GEO

Expression data from peritoneal macrophages stimulated with trehalose-6,6'-dimycolate (TDM)

Project description:Macrophage-inducible C-type lectin (Mincle, Clec4e) is a pathogen sensor that recognizes pathogenic fungi and Mycobactrium tuberculosis. We perfomed microarray analysis using peritoneal macrophages stimulated with TDM, a mycobacterial cell wall glycolipid that is known to be a Mincle ligand. Many chemokine and cytokine genes were upregulated in wildtype macrophages stimulated with TDM. Upregulation of these genes were completely abolishd in Mincle KO macrophages. Peritoneal macrophages from WT and Mincle KO mice were stimulated with TDM or vehicle for 24 h (3 samples each). Microarray analysis was performed using Affymetrix Mouse 430 2.0.

2013-11-14 | E-GEOD-52338 | biostudies-arrayexpress

Mus musculus

Project description:Card9 dependent activation of macrophages by TDB

| PRJNA107883 | ENA

Expression data from peritoneal macrophages stimulated with trehalose-6,6'-dimycolate (TDM)

2013-11-14 | GSE52338 | GEO

Transcription profiling of cumulus cell oocyte complexes isolated at different time points of ovulation to identify differentially expressed genes.

Project description:Cumulus-oocyte complexes were isolated at seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Experiment Overall Design: 2 eCG-primed (48h) pooled cumulus-oocyte complexes, 2 eCG-primed (48h) hCG-treated (8h) pooled cumulus-oocyte complexes, and 2 eCG-primed (48h) hCG-treated (16h) pooled cumulus-oocyte complexes replicates were analyzed

2007-11-06 | E-GEOD-4260 | biostudies-arrayexpress

RNA-seq data of TAGAP WT or KO BMDMs treated with Curdlan

Project description:Bone marrow derived macrophages (BMDMs) were isolated form WT or TAGAP-KO mice, followed by stimulation with Curdlan (10ug/ml) for 0 or 3 hours, and samples were collected and sent to BGI for RNA SEQ analysis.

2020-03-02 | GSE146156 | GEO

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