Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of cumulus cell oocyte complexes isolated at different time points of ovulation to identify differentially expressed genes.


ABSTRACT: Cumulus-oocyte complexes were isolated at seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Experiment Overall Design: 2 eCG-primed (48h) pooled cumulus-oocyte complexes, 2 eCG-primed (48h) hCG-treated (8h) pooled cumulus-oocyte complexes, and 2 eCG-primed (48h) hCG-treated (16h) pooled cumulus-oocyte complexes replicates were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: JoAnne Richards 

PROVIDER: E-GEOD-4260 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune-related genes: does this expand their role in the ovulation process?

Hernandez-Gonzalez Inmaculada I   Gonzalez-Robayna Ignacio I   Shimada Masayuki M   Wayne Chad M CM   Ochsner Scott A SA   White Lisa L   Richards JoAnne S JS  

Molecular endocrinology (Baltimore, Md.) 20060202 6


Ovulation is a complex process initiated by the preovulatory LH surge, characterized by cumulus oocyte complex (COC) expansion and completed by the release of a mature oocyte. Although many ovarian genes that impact ovulation have been identified, we hypothesized that genes selectively expressed in COCs would be overlooked by approaches using whole ovary or granulosa cell samples. RNA isolated from COCs collected from preovulatory follicles of equine chorionic gonadotropin (CG) primed mice and a  ...[more]

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