Unknown,Transcriptomics,Genomics,Proteomics

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Radiation-induced gene translation profiles reveal tumor type and cancer specific components

ABSTRACT: The goal of this study was to compare the radiation-induced gene translation profiles generated from human tumor cell lines that are treated with radiation. Keywords: stimulus or stress design A panel of cell lines included 5 gliomas, 4 pancreatic carcinomas, 3 breast carcinomas and 2 non-small cell lung carcinomas. In addition, radiation-induced gene translation profiles were generated for 4 normal human cell lines: a skin fibroblast (BJ), 2 lung fibroblasts (MRC5, MRC9) and mammary epithelial (MEC). Specifically, cell lines were exposed to 2 Gy or sham irradiated, polysome-bound RNA was isolated 6h later and subjected to microarray analyses. Each cell line was evaluated in biological replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Philip Tofilon

PROVIDER: E-GEOD-10547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Radiation-induced gene translation profiles reveal tumor type and cancer-specific components.

Kumaraswamy Sandhya S Chinnaiyan Prakash P Shankavaram Uma T UT Lü Xing X Camphausen Kevin K Tofilon Philip J PJ

Cancer research 20080501 10

The microarray analysis of total cellular RNA is a common method used in the evaluation of radiation-induced gene expression. However, profiling the cellular transcriptome does not take into account posttranscriptional processes that affect gene expression. To better define the genes whose expression is influenced by ionizing radiation, we used polysome-bound RNA to generate gene translation profiles for a series of tumor and normal cell lines. Cell lines were exposed to 2 Gy, polysome-bound RNA ...[more]

PMID: 18483266

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Dataset Information

Radiation-induced gene translation profiles reveal tumor type and cancer specific components

Publications

Radiation-induced gene translation profiles reveal tumor type and cancer-specific components.

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