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Mouse embryo or newborn liver or E15.5 embryo minus liver vs. pool of all samples

ABSTRACT: Transcriptional profiling of miRNA in embryonic and newborn mouse liver Keywords: miRNA, liver, newborn, embryonic Time course experiment with common control. Three biological replicates per time point.

ORGANISM(S): Mus musculus

SUBMITTER: Joshua Friedman

PROVIDER: E-GEOD-10602 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The microRNA-30 family is required for vertebrate hepatobiliary development.

Hand Nicholas J NJ Master Zankhana R ZR Eauclaire Steven F SF Weinblatt Daniel E DE Matthews Randolph P RP Friedman Joshua R JR

Gastroenterology 20081209 3

<h4>Background & aims</h4>The function of microRNA (miRNA) in liver development is unknown. To address this issue, we characterized miRNA expression in the embryonic mouse liver, performed functional miRNA analysis in zebrafish larvae, and identified novel hepatic miRNA targets.<h4>Methods</h4>Hepatic RNA isolated from mice at embryonic days 15.5, 18.5, and postnatal day 2 was hybridized to a mouse miRNA microarray. The microarray results were confirmed by Northern blot hybridization and quantit ...[more]

PMID: 19185580

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