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Stimulation of human umbilical venous endothelial cells (HUVECs) with bone morphogenetic protein 4 (BMP4).

ABSTRACT: Backround: Several microRNAs have been characterized to regulate the process of angiogenesis. Previously, bone morphogenetic protein 4 (BMP4) was shown to increase the proangiogenic activity of endothelial cells. Aim: In this project we investigated how the proangiogenic BMP4 effect is mediated by microRNAs. Experimental design: To investigate changes in the microRNA transcriptome in HUVECs a time course experiment (12, 18 and 24 h) was performed. HUVECs were kept in endothelial basal medium either with BMP4 (25 ng/ml) stimulation or buffer control (4 mM HCL with 0.1% bovine serum albumin). Comparison between stimulated vs. unstimulated cells at different time points. followed by a miRNA microarray analysis was performed.

ORGANISM(S): Homo sapiens

SUBMITTER: Jennifer Esser

PROVIDER: E-MTAB-4383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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