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Transcription profiling of human peripheral blood mononuclear cells isolated from LGL leukemia samples reveals role of sphingolipid signaling of cytotoxic lymphocytes

ABSTRACT: We used microarrays to expression profile peripheral blood mononuclear cells (PBMCs) from LGL leukemia patients and control subjects to identify survival pathways that render leukemic LGL resistant to activation induced cell death. Experiment Overall Design: Leukemic PBMC RNA from 30 patients was extracted for target preparation and hybridization onto Affymetrix microarrays. We also isolated PBMCs and PBMCs subjected to enrichment for CD8+ cells from control patients. RNA from these cells (naÃ¯ve or activated with phytohemagglutinin) was extracted for target preparation and hybridization onto Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Norman Lee

PROVIDER: E-GEOD-10631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular profiling of LGL leukemia reveals role of sphingolipid signaling of cytotoxic lymphocytes

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Transcription profiling of human CMV-stimulated CD69+, hCMV-stimulated CD69- and unstimulated monoclonal CD4+ T-LGL lymphocytes from peripheral blood samples from four TCRVbeta+/CD4+ T-LGL lymphocytosis patients

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