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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of Drosophila wild type and neoplastically transformed (lgl mutant) imaginal discs

ABSTRACT: In Drosophila, homozygosity for neoplastic tumor suppressors, like lethal giant larvae, lgl results in malignant transformation of all larval imaginal discs. To gain insight into the molecular players involved in fly tumorigenesis we undertook genome-wide transcriptional profiling of neoplastically transformed wing imaginal discs of lgl mutants. Wild type (Canton S) wing imaginal discs served as control. The transcriptional profile, for instance, revealed the misregulation of JNK and JAK-STAT signaling which is implicated in epithelial transformation in Drosophila. These transcriptional profiles, therefore, provide a useful resource for identification of genes/pathways that are functionally relevant for carcinogenesis.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Subhabrata Pal

PROVIDER: E-MEXP-2920 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of neoplastically transformed and wild type wing imaginal discs of Drosophila

Project description:In Drosophila, homozygosity for tumor suppressors, like lethal giant larvae, lgl, results in neoplastic transformation of imaginal disc epithelium - characterized by loss of their apico-basal polarity, unrestricted growth and invasion into neighboring tissues/organs. In genetic mosaics, however, lgl mutant clones are eliminated shortly after their initiation by cell competition. On the other hand, lgl mutant clones generated in Minute (M) genetic background, which compromises cell competition from the neighboring wild type cells, display clonal progression and tissue invasion. To gain insight into the molecular players of fly tumorigensis we undertook genome-wide transcriptional profiling of neoplastically transformed mosaic wing imaginal discs where lgl- clones were induced in M genetic background. Mosaic wing imaginal discs carrying wild type (lgl+) clones, also generated in M genetic background, served as controls. Transcriptional profile revealed up-regulation of a number of signaling pathways, such as Hippo, Wnt, TGF-beta or EGFR. Transcriptional profile of lgl- mosaic imaginal discs therefore provides a useful resource for identification of genes/pathways that are functionally relevant for carcinogenesis

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