Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of the transcriptomes of hmgb1 vs Col-0


ABSTRACT: Testing the consequences of the absence of the chromatin-associated HMGB1 protein on the transcriptome in Arabidopsis. Keywords: Comparison of mutant and wildtype samples. Four replicate RNA extractions were performed of each genotype using independent pools of plants. hmgb1#1 (Cy5) vs. Col-0#1 (Cy3) Col-0#2 (Cy5) vs. hmgb1#2 (Cy3) dye-swap hmgb1#3 (Cy5) vs. Col-0#3 (Cy3) Col-0#5 (Cy5) vs. hmgb1#4 (Cy3) dye-swap Samples were hybridized in two dye-swaps. The data were normalized over all 4 hybridizations to obtain one, single log-ratio(Sample/Reference). The raw data files of all four of the hybridizations are attached to the Sample.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Joke Allemeersch 

PROVIDER: E-GEOD-10638 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The expression level of the chromatin-associated HMGB1 protein influences growth, stress tolerance, and transcriptome in Arabidopsis.

Lildballe Dorte Launholt DL   Pedersen Dorthe S DS   Kalamajka Rainer R   Emmersen Jeppe J   Houben Andreas A   Grasser Klaus D KD  

Journal of molecular biology 20080916 1


High mobility group (HMG) proteins of the HMGB family are small and relatively abundant chromatin-associated proteins. As architectural factors, the HMGB proteins are involved in the regulation of transcription and other DNA-dependent processes. We have examined Arabidopsis mutant plants lacking the HMGB1 protein, which is a typical representative of the plant HMGB family. In addition, our analyses included transgenic plants overexpressing HMGB1 and mutant plants that were transformed with the H  ...[more]

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