Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis to identify novel genes regulated by Cholera toxin in bone marrow-derived dendritic cells (BMDCs)

ABSTRACT: The mechanisms by which dendritic cells (DCs) induce differentiation of naÃ¯ve CD4+ T cells along the Th2 lineage is not well understood given that DCs themselves do not produce IL-4. In the present study, we undertook a microarray approach to identify genes involved in the induction of Th2 differentiation by DCs treated with a Th2-skewing adjuvant cholera toxin (CT). In the microarray analysis, murine bone marrow derived immature DCs were treated with CT. Of particular interest was tthe significant upregulation of the expression of c-kit. The upregulation of c-kit on DCs is critical for the induction of a Th2 response. Keywords: cholera toxin, bone marrow derived dendritic cells, gene expression array-based (RNA / spotted DNA/cDNA) Murine bone marrow cells were cultured in the presence of GM-CSF (10 ng/ml) for 6 days . On day 6 the cells were harvested and purified using magnetically labeled anti mouse CD11c+ beads . The DCs were stimulated with CT (1 ug/ml) for 24 hours and the RNA was isolated.

ORGANISM(S): Mus musculus

SUBMITTER: Anuradha Ray

PROVIDER: E-GEOD-10815 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma.

Krishnamoorthy Nandini N Oriss Timothy B TB Paglia Melissa M Fei Mingjian M Yarlagadda Manohar M Vanhaesebroeck Bart B Ray Anuradha A Ray Prabir P

Nature medicine 20080504 5

Dendritic cells (DCs) are integral to the differentiation of T helper cells into T helper type 1 T(H)1, T(H)2 and T(H)17 subsets. Interleukin-6 (IL-6) plays an important part in regulating these three arms of the immune response by limiting the T(H)1 response and promoting the T(H)2 and T(H)17 responses. In this study, we investigated pathways in DCs that promote IL-6 production. We show that the allergen house dust mite (HDM) or the mucosal adjuvant cholera toxin promotes cell surface expressio ...[more]

PMID: 18454155

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Dataset Information

Microarray analysis to identify novel genes regulated by Cholera toxin in bone marrow-derived dendritic cells (BMDCs)

Publications

Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma.

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