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Transcriptomic regulation during meiosis (Spo11Y135F mutant)

ABSTRACT: We studied the meiotic regulation of transcription in the mutant Spo11Y135F (deficient for the formation of meiotic double-strand breaks), by comparing the mRNA level at time t=1h to t=8h after meiosis induction, to the mRNA level at time t=0h. Keywords: yeast meiotic time course, transcriptome For each time point, we measured the level of mRNA extracted at the time point to a mRNA meiotic mix composed of mRNA extracted at each time point. We normalize this ratio to the ratio measured at time t=0h and transform into log2ratios. The experiment is carried out in duplicates, from two independant cultures.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-10947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic regulation during meiosis

Project description:We studied the meiotic regulation of transcription, by comparing the mRNA level at time t=1h to t=8h after meiosis induction, to the mRNA level at time t=0h. Keywords: yeast meiotic time course, transcriptome For each time point, we measured the level of mRNA extracted at the time point to a mRNA meiotic mix composed of mRNA extracted at each time point. We normalize this ratio to the ratio measured at time t=0h and transform into log2ratios. The experiment is carried out in triplicates, from three independant cultures.

2008-10-31 | E-GEOD-10944 | biostudies-arrayexpress

Transcriptomic regulation during meiosis (Clb5Delta-Clb6Delta mutant)

Project description:We studied the meiotic regulation of transcription in the mutant Clb5Delta-Clb6Delta (deficient for the meiotic replication), by comparing the mRNA level at time t=1h to t=8h after meiosis induction, to the mRNA level at time t=0h. Keywords: yeast meiotic time course, transcriptome For each time point, we measured the level of mRNA extracted at the time point to a mRNA meiotic mix composed of mRNA extracted at each time point. We normalize this ratio to the ratio measured at time t=0h and transform into log2ratios.

2008-10-31 | E-GEOD-10948 | biostudies-arrayexpress

Transcriptomic regulation and methylation of the Lysine4 of Histone H3 during meiosis

Project description:This SuperSeries is composed of the following subset Series: GSE10836: Meiotic time course of Histone H3 occupancy GSE10837: Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3) in a mutant Spo11F GSE10838: Meiotic time course of Histone H3 occupancy in a mutant Spo11F GSE10839: Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3) in a mutant clb5Delta-clb6Delta GSE10840: Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3) GSE10944: Transcriptomic regulation during meiosis GSE10947: Transcriptomic regulation during meiosis (Spo11Y135F mutant) GSE10948: Transcriptomic regulation during meiosis (Clb5Delta-Clb6Delta mutant) GSE12879: Meiotic DNA double strand breaks in wild-type and set1 cells Keywords: SuperSeries Refer to individual Series

2008-10-31 | E-GEOD-11004 | biostudies-arrayexpress

Transcriptomic regulation during meiosis

2008-11-01 | GSE10944 | GEO

Transcriptomic regulation during meiosis (Clb5Delta-Clb6Delta mutant)

2008-11-01 | GSE10948 | GEO

Transcriptomic regulation during meiosis (Spo11Y135F mutant)

Project description:We studied the meiotic regulation of transcription in the mutant Spo11Y135F (deficient for the formation of meiotic double-strand breaks), by comparing the mRNA level at time t=1h to t=8h after meiosis induction, to the mRNA level at time t=0h. Keywords: yeast meiotic time course, transcriptome

2008-11-01 | GSE10947 | GEO

Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3)

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3K4me3 by ChIP-chip. Keywords: ChIP-chip, meiotic time course, histone modification Strain ORD7339 meiotic time-course from 0 to 6h. Immunoprecipitation with rabbit polyclonal anti-triMe H3K4 (Abcam #Ab8580).

2010-05-17 | E-GEOD-10840 | biostudies-arrayexpress

Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3) in a mutant Spo11F

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3K4me3 by ChIP-chip in a mutant deficient for DSB formation. Keywords: ChIP-chip, meiotic time course, histone modification Strain ORD7341 meiotic time-course from 0 to 6h. Immunoprecipitation with rabbit polyclonal anti-triMe H3K4 (Abcam #Ab8580).

2010-05-17 | E-GEOD-10837 | biostudies-arrayexpress

Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3) in a mutant clb5Delta-clb6Delta

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3K4me3 by ChIP-chip in a mutant deficient for DSB formation in a mutant clb5Delta-clb6Delta Keywords: ChIP-chip, meiotic time course, histone modification Strain ORD6830 meiotic time-course from 0 to 6h. Immunoprecipitation with rabbit polyclonal anti-triMe H3K4 (Abcam #Ab8580).

2010-05-17 | E-GEOD-10839 | biostudies-arrayexpress

A shotgun proteomics reference map for the alkali-sensitive covalently-linked proteins of the cell wall of A. fumigatus during specific stages of early fungal development (dormant conidia, isotropically expanding conidia, early germ tube emergence and young hyphae).

Project description:Four different developmental time points representing the Afu development from dormant conidia into young hyphae (0h, 4h, 6h, 8h) were chosen. Cell walls of the Afu samples were extracted using denaturing methods followed by isolation of solubilized proteins. Samples were processed using filter aided sample preparation (FASP) / trypsin digestion. The peptides were analyzed by one-dimensional LC (capillary C18 nano-Eflow mode) on-line with a nano-ESI source and tandem MS (LTQ ion trap).

2016-12-20 | PRD000567 | Pride

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