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Transcription profiling of human memory T cell subsets from peripheral blood.

ABSTRACT: Microarray deconvolution is a technique for quantifying the relative abundance of constituent cells in a mixture based on that mixture's microarray signature and the signatures of the purified constituents. It has been applied to yeast and other systems but not to blood samples. Here we test the ability of this technique to determine the fractions of subsets of memory T cells in peripheral blood mononuclear cell (PBMC) samples. Experiment Overall Design: PBMC samples from several donors were split. One portion of each PBMC sample was set aside, while the remainder of each was sorted into naive, central memory, and effector memory T cell subsets.

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Abbas

PROVIDER: E-GEOD-11057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deconvolution of blood microarray data identifies cellular activation patterns in systemic lupus erythematosus.

Abbas Alexander R AR Wolslegel Kristen K Seshasayee Dhaya D Modrusan Zora Z Clark Hilary F HF

PloS one 20090701 7

Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease with a complex spectrum of cellular and molecular characteristics including several dramatic changes in the populations of peripheral leukocytes. These changes include general leukopenia, activation of B and T cells, and maturation of granulocytes. The manifestation of SLE in peripheral blood is central to the disease but is incompletely understood. A technique for rigorously characterizing changes in mixed populations of cells, ...[more]

PMID: 19568420

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