Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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MRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected


ABSTRACT: As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2 & miR-1, and +FLAG-Ago2 & miR-124. Set of arrays that are part of repeated experiments Compound Based Treatment: mock transfected Keywords: Biological Replicate Biological Replicate Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Hogan 

PROVIDER: E-GEOD-11079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance.

Hendrickson David G DG   Hogan Daniel J DJ   Herschlag Daniel D   Ferrell James E JE   Brown Patrick O PO  

PloS one 20080507 5


microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understanding of miRNA function is inferred largely from studies of the effects of miRNAs on steady-state mRNA levels and from seed match conservation and context in putative targets. Here we have taken a more direct approach to these issues by comprehensively assessing the miRNAs and mRNAs that are physically associated with Argon  ...[more]

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