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Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA

ABSTRACT: miRNAs were enriched from HEK293T cells using the ambion FLASHPAGE fractionater after mock transfection, Ago2 transfection, and FLAG-Ago2 IP. miRNAs were labeled and hybridized: Ago2 transfected vs. mock transfection and Ago2 transfected vs. Ago2 IPs. Set of arrays that are part of repeated experiments Keywords: Biological Replicate Biological Replicate Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Hogan

PROVIDER: E-GEOD-11081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance.

Hendrickson David G DG Hogan Daniel J DJ Herschlag Daniel D Ferrell James E JE Brown Patrick O PO

PloS one 20080507 5

microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understanding of miRNA function is inferred largely from studies of the effects of miRNAs on steady-state mRNA levels and from seed match conservation and context in putative targets. Here we have taken a more direct approach to these issues by comprehensively assessing the miRNAs and mRNAs that are physically associated with Argon ...[more]

PMID: 18461144

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Expression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock

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2008-04-07 | E-GEOD-11080 | biostudies-arrayexpress

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2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress

Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance

Project description:This SuperSeries is composed of the following subset Series: GSE11079: mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected GSE11080: Expression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock GSE11081: miRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA Keywords: SuperSeries Refer to individual Series

2008-04-07 | E-GEOD-11082 | biostudies-arrayexpress

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2009-09-08 | E-GEOD-12827 | biostudies-arrayexpress

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