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Array-based comparative genomic hybridisation analysis at 1Mb resolution of 44 pilocytic astrocytomas

ABSTRACT: Pilocytic astrocytomas (PAs), WHO Grade I, are one of the most frequently occurring childhood brain tumors. We have used microarray comparative genomic hybridization (aCGH) at 1Mb resolution to study copy number changes in a series of PAs (n=44). Single hybridization per case. 44 pilocytic astrocytomas WHO grade I were analyzed. Target (tumor) labelled with Cy5 and reference with Cy3. Mixture of 20 normal male or female genomic DNA was used in sex-mismatched hybridization.

ORGANISM(S): Homo sapiens

SUBMITTER: David Jones

PROVIDER: E-GEOD-11263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic analysis of pilocytic astrocytomas at 0.97 Mb resolution shows an increasing tendency toward chromosomal copy number change with age.

Jones David T W DT Ichimura Koichi K Liu Lu L Pearson Danita M DM Plant Karen K Collins V Peter VP

Journal of neuropathology and experimental neurology 20061101 11

Brain tumors are the most common solid tumors of childhood, accounting for over 20% of cancers in children under 15 years of age. Pilocytic astrocytomas (PAs), World Health Organization grade I, are one of the most frequently occurring childhood brain tumors, yet little is known about genetic changes characterizing this entity. We have used microarray comparative genomic hybridization at 0.97 Mb resolution to study a series of PAs (n = 44). No copy number abnormality was seen in 64% of cases at ...[more]

PMID: 17086101

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