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Drosophila melanogaster small RNA sequence from 14 libraries

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Wei-Jen Chung

PROVIDER: E-GEOD-11624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Endogenous RNA interference provides a somatic defense against Drosophila transposons.

Chung Wei-Jen WJ Okamura Katsutomo K Martin Raquel R Lai Eric C EC

Current biology : CB 20080522 11

<h4>Background</h4>Because of the mutagenic consequences of mobile genetic elements, elaborate defenses have evolved to restrict their activity. A major system that controls the activity of transposable elements (TEs) in flies and vertebrates is mediated by Piwi-interacting RNAs (piRNAs), which are approximately 24-30 nucleotide RNAs that are bound by Piwi-class effectors. The piRNA system is thought to provide primarily a germline defense against TE activity.<h4>Results</h4>Here, we describe a ...[more]

PMID: 18501606

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