Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster small RNA sequence: cell lines and imaginal disc

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster S2 and KC cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Wei-Jen Chung

PROVIDER: E-GEOD-10794 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs.

Okamura Katsutomo K Chung Wei-Jen WJ Ruby J Graham JG Guo Huili H Bartel David P DP Lai Eric C EC

Nature 20080507 7196

In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology. Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes (hpRNAs) pro ...[more]

PMID: 18463630

Similar Datasets

Drosophila melanogaster small RNA sequence from 14 libraries

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

2008-06-01 | E-GEOD-11624 | biostudies-arrayexpress

Drosophila melanogaster late embryo, larval, pupal and adult head small RNA libraries

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. Total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster (Canton S) wandering late embryos, first instar larvae, third instar larvae or from 2-4 day old pupae and adult female heads or male heads. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2008-09-30 | E-GEOD-12840 | biostudies-arrayexpress

Drosophila melanogaster small RNA sequence: Kc167 cells (Rubin lab)

Project description:modENCODE_submission_2374 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Cell Line: Kc-Rubin; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Genotype: se/e; Sex: Female; EXPERIMENTAL FACTORS: Cell Line Kc-Rubin; Replicate type: biological

2009-04-30 | E-GEOD-15899 | biostudies-arrayexpress

Drosophila melanogaster small RNA sequence: female heads

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster female head. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt Quality scores in the supplementary file for GSM240749 are undefined. Quality of the bases assessed by (1) identifying for the sequenced linker, which is a known sequence, and (2) mapping the clipped sequence to the genome and taking only perfect hits.

2008-04-22 | E-GEOD-10790 | biostudies-arrayexpress

small RNA from S2 cells (S2-NP)

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2 cells obtained from Norbert Perrimon's lab. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2009-04-30 | E-GEOD-14849 | biostudies-arrayexpress

High-throughput sequencing of Drosophila melanogaster small RNAs from S2R+ cells

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from S2R+ cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2R+ cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2008-12-04 | E-GEOD-13679 | biostudies-arrayexpress

small RNA from Drosophila melanogaster - cell line Kc167

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from Kc167 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster Kc167 cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2009-04-30 | E-GEOD-15897 | biostudies-arrayexpress

Drosophila melanogaster S2-GMR cells, second biological replicate

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2 cells obtained from Gerald Rubin's lab. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2009-01-22 | E-GEOD-14488 | biostudies-arrayexpress

small RNA from Drosophila melanogaster - adult bodies

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from imaginal disc / brain, female body and male body. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster adult bodies. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

2009-04-30 | E-GEOD-15898 | biostudies-arrayexpress

Drosophila melanogaster Canton-S small RNA sequence: Adult Female Body

Project description:modENCODE_submission_2555 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Developmental Stage: Adult female; Genotype: wild type; Sex: Female; EXPERIMENTAL FACTORS: Strain Canton S; Tissue Female body; Replicate type: biological;

2012-08-08 | E-GEOD-40015 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data