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Drosophila melanogaster small RNA sequence: cell lines and imaginal disc

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster S2 and KC cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Wei-Jen Chung

PROVIDER: E-GEOD-10794 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs.

Okamura Katsutomo K Chung Wei-Jen WJ Ruby J Graham JG Guo Huili H Bartel David P DP Lai Eric C EC

Nature 20080507 7196

In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology. Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes (hpRNAs) pro ...[more]

PMID: 18463630

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