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Transcription profiling of mouse IFN producing killer dendritic cells

ABSTRACT: IKDCs have been newly characterized in mouse and shared functional and phenotypic properties of NK cells and dendritic cells. To formaly distinguish them from NK cells, we have performed a gene expression profile analysis of splenic and LN cell sorted IKDC and compared them with CDC and NK.We are looking for gene or group of genes specifically expressed by IKDCs and which would allow us to specifically isolate and characterize them. Experiment Overall Design: IKDC, NK and CDC were cell-sorted from spleen and lymph node (LN) of naÃ¯ve BALB/c mice (6-8 weeks).

ORGANISM(S): Mus musculus

SUBMITTER: Franck Housseau

PROVIDER: E-GEOD-11918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular characterization of murine IFN producing killer dendritic cells

Project description:IKDCs have been newly characterized in mouse and shared functional and phenotypic properties of NK cells and dendritic cells. To formaly distinguish them from NK cells, we have performed a gene expression profile analysis of splenic and LN cell sorted IKDC and compared them with CDC and NK.We are looking for gene or group of genes specifically expressed by IKDCs and which would allow us to specifically isolate and characterize them. Keywords: Freshly isolated cells

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Antiviral State of CD1c+ cDC associates with increased Natural Killer maturation and activation in Sjögren´s Syndrome

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Transcription profiling of mouse resting and IL-15 activated natural killer cells

Project description:Murine NK cells were compared at rest and following 24 hours of IL-15 stimulation for their mRNA expression profiles on the Affymetrix MOE430_2 microarray platform. Additional comparators included resting bulk splenocytes. Experiment Overall Design: Three pairs of flow sorted murine NK cells (C57Bl/6) were analzyed at rest or after 24 hours of IL-15 stimulation. Three biological replicates of each condition (resting or IL-15) are included in this anlaysis. In addition, bulk resting splenocytes were used (2 biological replicates with 2 chip replicates for a total of 4 replicates).

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Natural killer receptor-positive innate lymphoid cells

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Transcription profiling by array of human CD56brightCD16-, CD56dimNKG2A+ and CD56dimNKG2A- natural killer cell subsets

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2010-08-10 | E-MEXP-2798 | biostudies-arrayexpress

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Project description:Previous reports have defined three subsets of mouse NK cells on the basis of the expression of CD27 and CD11b. The developmental relationship between these subsets was unclear. To address this issue, we evaluated the overall proximity between mouse NK cell subsets defined by CD27 and CD11b expression using pangenomic gene expression profiling. The results suggest that CD27+CD11b-, CD27+CD11b+ and CD27-CD11b+ correspond to three different intermediates stages of NK cell development. Experiment Overall Design: Spleen cells from RAG-/- mice have been isolated and stained with anti-NK1.1, anti CD27 and anti CD11b antibodies. NK1.1+ cells were sorted into CD27+ CD11b-, CD27+ CD11b+ and CD27- CD11b+ subsets by flow cytometry. There are two independent replicates for each sample. Total RNA was extracted with the RNeasy microkit (Qiagen) and gene expression profiles were performed according to manufacturer instructions (Affymetrix mouse 430 2.0).

2009-03-21 | E-GEOD-13229 | biostudies-arrayexpress

Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis

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Transcriptome analysis of human Natural Killer (NK) cells expressing a RUNX2-specific shRNA.

Project description:Research has already gained much insight into the roles of various members of the RUNX family of transcription factors in the development of human T- and B-cells, however the importance in human NK cell development remains unclear. Therefore, we transduced a shRNA that specifically binds to all known RUNX2 isoforms in human umbilical cord blood-derived hematopoietic progenitor cells (HPC) and cultured them in vitro in an NK cell differentiation model. On day 21 of culture (CD56+CD94+) NK cells from shRNA and control cultures were sorted, after which mRNA was isolated and transcriptome analysis was performed by RNA sequencing. Evaluation of the transcriptome in these NK cells could reveal the molecular mechanisms of RUNX2.

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In vivo G-CSF Treatment Activates GR-SOCS1 Axis Suppressing IFN-γ Secretion by Natural Killer Cells [CUT&TAG]

Project description:Natural killer (NK) cells are lymphocytes of the innate immune system that are involved in controlling tumors or microbial infections through the production of interferon gamma (IFN-γ). Granulocyte-colony stimulating factor (G-CSF) inhibits IFN-γ secretion by NK cells, but the mechanism underlying this effect remains unclear. Here, by comparing the multi-omics profiles of human NK cells before and after in vivo G-CSF treatment, we identified a pathway that was activated in response to G-CSF treatment, which suppressed IFN-γ secretion in NK cells. Specifically, our integrative genomic strategy revealed glucocorticoid receptor (GR) activation in NK cells that mediated the genomic response to G-CSF treatment. Activated GRs can inhibit secretion of IFN-γ by promoting interactions between suppressor of cytokine signaling 1 (SOCS1) promoter and enhancer, as well as increase the expression of SOCS1. Experiments in mice confirmed that G-CSF in vivo treatment significantly down-regulated IFN-γ secretion and up-regulated GR and SOCS1 expression in NK cells. In addition, GR blockade (RU486) significantly reversed the effects of G-CSF, demonstrating that GR up-regulates SOCS1 and inhibits the production of IFN-γ by NK cells.

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