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Transcription profiling of mouse resting and IL-15 activated natural killer cells

ABSTRACT: Murine NK cells were compared at rest and following 24 hours of IL-15 stimulation for their mRNA expression profiles on the Affymetrix MOE430_2 microarray platform. Additional comparators included resting bulk splenocytes. Experiment Overall Design: Three pairs of flow sorted murine NK cells (C57Bl/6) were analzyed at rest or after 24 hours of IL-15 stimulation. Three biological replicates of each condition (resting or IL-15) are included in this anlaysis. In addition, bulk resting splenocytes were used (2 biological replicates with 2 chip replicates for a total of 4 replicates).

ORGANISM(S): Mus musculus

SUBMITTER: Rekha Meyer

PROVIDER: E-GEOD-7764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Acquisition of murine NK cell cytotoxicity requires the translation of a pre-existing pool of granzyme B and perforin mRNAs.

Fehniger Todd A TA Cai Sheng F SF Cao Xuefang X Bredemeyer Andrew J AJ Presti Rachel M RM French Anthony R AR Ley Timothy J TJ

Immunity 20070531 6

Although activated murine NK cells can use the granule exocytosis pathway to kill target cells immediately upon recognition, resting murine NK cells are minimally cytotoxic for unknown reasons. Here, we showed that resting NK cells contained abundant granzyme A, but little granzyme B or perforin; in contrast, the mRNAs for all three genes were abundant. Cytokine-induced in vitro activation of NK cells resulted in potent cytotoxicity associated with a dramatic increase in granzyme B and perforin, ...[more]

PMID: 17540585

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