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Expression data from Emx2 wildtype and knockout olfactory epithelium

ABSTRACT: Emx2 is a homeobox transcription factor that plays a critical role in development. Olfactory sensory neuron axons from Emx2 knockout mice fail to innervate their target tissue, the olfactory bulb. Homeobox transcription factors may also play an important role in olfactory receptor expression. We used microarrays to analyze differences in mRNA abundance in the olfactory epithelium of Emx2 wildtype and knockout embryonic day 18.5 mice. Keywords: Knockout-Wildtype comparision Total RNA from 9 Emx2 wildtype and 9 Emx2 knockout mice was analyzed. Equal amounts of RNA from 3 animals was pooled to create one biological sample, 3 pools per genotype, 6 chips total. RNA was hybridized to the Affymetrix Mouse Exon 1.0 ST array.

ORGANISM(S): Mus musculus

SUBMITTER: Jeremy McIntyre

PROVIDER: E-GEOD-12135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Emx2 stimulates odorant receptor gene expression.

McIntyre Jeremy C JC Bose Soma C SC Stromberg Arnold J AJ McClintock Timothy S TS

Chemical senses 20081014 9

The mechanisms selecting a single odorant receptor (OR) gene for expression in each olfactory sensory neuron (OSN) establish an OR expression pattern critical for odor discrimination. These mechanisms are largely unknown, but putative OR promoters contain homeodomain-like sites, implicating homeobox transcription factors such as Emx2. At embryonic day 18.5, expression of 49-76% of ORs was decreased in mice lacking Emx2, depending on the metric used. The decreases were due to fewer OSNs expressin ...[more]

PMID: 18854508

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Expression data from Emx2 wildtype and knockout olfactory epithelium

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