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Transcription profiling of human alveolar macrophages in response to P. aeruginosa and B. cepacia infections

ABSTRACT: To determine if differences in the severity of pulmonary infection in cystic fibrosis been seen in late isolates od Pseudomonas aeruginosa and Burkholderia cepacia are associated with differences in the initial repsonse of alveolar macrophages (AM) to these pathogens, we assessed gene expression changes in human AM in response to infection with a laboratoty strain, early and late clinical isolates of P. aeruginosa, and B. cepacia. Experiment Overall Design: Alveolar macrophages were obtained from bronchoalveolar lavage. Experiment Overall Design: Two clinical strains isolated from the sputum of an individual with CF, AD2A and AD15B (provided by J. Burns, University of Washington, Seattle). AD2A is an early clinical isolate, and AD15B is a late clinical isolate; both were derived from the same individual.

ORGANISM(S): Homo sapiens

SUBMITTER: Jacqueline Salit

PROVIDER: E-GEOD-12245 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:To determine if differences in the severity of pulmonary infection in cystic fibrosis been seen in late isolates od Pseudomonas aeruginosa and Burkholderia cepacia are associated with differences in the initial repsonse of alveolar macrophages (AM) to these pathogens, we assessed gene expression changes in human AM in response to infection with a laboratoty strain, early and late clinical isolates of P. aeruginosa, and B. cepacia. Keywords: Comparison of gene expression in alveolar macrophages of normal non-smokers and normal smokers.

2008-07-26 | GSE12245 | GEO

Pulmonary ketogenesis promotes tolerance to bacterial infection

Project description:Tolerance is a major defense strategy against infection. During the host response to pathogens, tolerance restricts inflammatory damage to tissues, maintaining the long-term integrity of organs. The mechanisms that establish tolerance are poorly understood. We analyzed pulmonary isolates of Pseudomonas aeruginosa that evolved to coexist with tolerant hosts and found that these opportunistic pathogens facilitate tolerance by stimulating ketogenesis. Ketone body production in the airway limits the accumulation of detrimental factors that injure the lung, such as inflammatory cytokines and effector phagocytes. Although ketones are typically synthesized in the liver, in situ metabolo-transcriptomic studies revealed that P. aeruginosa drives their generation in the lung by co-opting the metabolism of alveolar macrophages, which are pulmonary cells of hepatic origin. This phenomenon was restricted to clinical isolates and not observed with laboratory strains of P. aeruginosa , confirming the unique metabolo-tolerogenic properties of ESKAPE pathogens adapted to the human lung.

2023-10-12 | GSE203352 | GEO

Expression data from Burkholderia cepacia IST408 bceE and bceF mutants

Project description:The bacterial tyrosine (BY) kinase family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We have previously shown that the BceF protein from Burkholderia cepacia IST408 belongs to this BY kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation in the cell by this kinase protein. In order to examine this, we performed a comparative transcriptome profile between the bceF mutant and the wild-type B. cepacia IST408. As a control, the transcriptome of another exopolysaccharide-deficient mutant, which had a mutation in the bceE gene, was also determined. For expression profiling, overnight cultures of the Burkholderia cepacia IST408 wild-type strain and the isogenic mutants bceE::Tp and bceF::Tp grown in LB medium were diluted to an initial OD640 nm of 0.1 into S medium. Triplicate samples were cultured at 30ÂºC with 250 r.p.m. agitation for 12 h, and RNA was extracted from the bacterial isolates. DNA-based probe-selection was performed using a CEL file derived from a hybridization of genomic DNA, and Xspecies DNA hyb CDF batchmaker v3.2, available here: http://affymetrix.arabidopsis.info/xspecies/. An optimal signal cutoff of 80 and minimum number of probes per probe set of 7 were determined empirically, resulting in the masking of 24223 of the original 226576 probes on the array and the exclusion of 156 probe sets. The new cdf file created (Bcc1sa520656F_Xspecies.CEL80.cdf) was used in DNA-Chip Analyzer 2008 for the subsequent gene expression analyses.

2012-12-01 | E-GEOD-38183 | biostudies-arrayexpress

Expression data from Burkholderia cepacia IST408 bceE and bceF mutants

2012-12-01 | GSE38183 | GEO

Pseudomonas aeruginosa

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Gene expression in P. aeruginosa cystic fibrosis isolates grown on Nematode Growth Medium

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Burkholderia cepacia

Project description:Burkholderia cepacia Genome sequencing

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Burkholderia cepacia

Project description:Burkholderia cepacia Genome sequencing

| PRJNA863394 | ENA

Burkholderia cepacia strain:JK6 | isolate:JK6

Project description:Burkholderia cepacia strain:JK6 | isolate:JK6 Genome sequencing

| PRJNA1141197 | ENA

Gene expression in P. aeruginosa cystic fibrosis isolates grown on Nematode Growth Medium

2011-12-31 | E-GEOD-33275 | biostudies-arrayexpress