Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Normal rat diaphragm vs sternohyoid

ABSTRACT: Normal young adult Sprague Dawley rats (male) 6 diaphragm muscle samples are compared with 6 sternohyoid muscle samples -- each sample is one muscle from one rat

ORGANISM(S): Rattus norvegicus

SUBMITTER: Erik van Lunteren

PROVIDER: E-GEOD-12282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Differential expression of lipid and carbohydrate metabolism genes in upper airway versus diaphragm muscle.

van Lunteren Erik E Spiegler Sarah S Moyer Michelle M

Sleep 20100301 3

<h4>Study objectives</h4>Contractile properties of upper airway muscles influence upper airway patency, an issue of particular importance for subjects with obstructive sleep apnea. Expression of genes related to cellular energetics is, in turn, critical for the maintenance of contractile integrity over time during repetitive activation. We tested the hypothesis that sternohyoid has lower expression of genes related to lipid and carbohydrate energetic pathways than the diaphragm.<h4>Methods</h4>S ...[more]

PMID: 20337195

Similar Datasets

Zucker Diabetic Fatty (ZDF) Rat Study - Diaphragm vs. Sternohyoid

Project description:Type 2 diabetes differs from type 1 diabetes in its pathogenesis. Type 1 diabetic diaphragm has altered gene expression which includes lipid and carbohydrate metabolism, ubiquitination and oxidoreductase activity. The objectives of the present study were to assess respiratory muscle gene expression changes in type 2 diabetes and to determine whether they are greater for the diaphragm than an upper airway muscle. Diaphragm and sternohyoid muscle from Zucker diabetic fatty (ZDF) rats were analyzed with Affymetrix gene expression arrays. The two muscles had 97 and 102 genes, respectively, with at least ±1.5-fold significantly changed expression with diabetes, and these were assigned to gene ontology groups based on over-representation analysis. Several significantly changed groups were common to both muscles, including lipid metabolism, carbohydrate metabolism, muscle contraction, ion transport and collagen, although the number of genes and the specific genes involved differed considerably for the two muscles. In both muscles there was a shift in metabolism gene expression from carbohydrate metabolism toward lipid metabolism, but the shift was greater and involved more genes in diabetic diaphragm than diabetic sternohyoid muscle. Groups present in only diaphragm were blood circulation and oxidoreductase activity. Groups present in only sternohyoid were immune & inflammation and response to stress & wounding, with complement genes being a prominent component. In conclusion, type 2 diabetes-induced gene expression changes in respiratory muscles has both similarities and differences relative to previous data on type 1 diabetes gene expression. Furthermore, the diabetic alterations in gene expression differ between diaphragm and sternohyoid. 18-week-old Zucker diabetic fatty rats: 6 normal and 5 diabetic diaphragms and sternohyoids.

2014-05-12 | E-GEOD-21791 | biostudies-arrayexpress

Laminin-deficient muscular dystrophy, dy/dy diaphragm

Project description:Laminin (merosin) deficient muscular dystrophy in dy/dy mouse diaphragm muscle, 8 weeks old Keywords: muscle, muscular dystrophy, laminin, merosin, diaphragm, mouse

2006-03-16 | GSE3252 | GEO

Transcription profiling of rat treated with streptozotocin or buffer alone at age of 8 weeks, diaphragm muscle

Project description:8 week old rats injected with streptozotocin or buffer alone at age of 8 weeks, diaphragm muscle obtained at age of 12 weeks (thus animals were diabetic for 4 weeks) Experiment Overall Design: Three control rats (normal diaphragm #1, 2, 3) Experiment Overall Design: Three diabetic rats (diabetic diaphragm #1, 2, 3)

2009-11-28 | E-GEOD-4653 | biostudies-arrayexpress

Diaphragm in Diabetes

2009-11-13 | GSE4653 | GEO

Gene expression profile in the diaphragm following contractile inactivity during thoracic surgery

Project description:Recent work revealed the development of marked diaphragm muscle fiber weakness during two hours of thoracic surgery. This loss of muscle fiber function was not part of a generalized muscle weakness as function of the non-respiratory latissimus dorsi muscle was preserved. To disentangle the molecular processes that might underlie the development of diaphragm muscle fiber weakness during thoracic surgery we studied changes in the gene expression profile. Analyzed were diaphragm samples at t0 vs t2 and latissimus dorsi at t0 vs t2. In total 8 Diaphragm samples and 6 latissimus dorsi samples were analyzed.

2013-07-03 | E-GEOD-30658 | biostudies-arrayexpress

Gene expression profile in the diaphragm following contractile inactivity during thoracic surgery

2013-07-03 | GSE30658 | GEO

Zucker Diabetic Fatty (ZDF) Rat Study - Diaphragm vs. Sternohyoid

2014-05-12 | GSE21791 | GEO

Gate-Keeping transcription through histone H3 K42, a novel site of methylation in the yeast nucleosome core

Project description:A new site of methylation was identified on histone H3 K42 in Saccharomyces cerevisiae. Mutations were engineered at this site to mimic either a constitutively modified state, K42L, or a constitutively unmodified state, K42Q in addition to an alanine substitution. K42A. The effects of these mutations on global transcription was monitored in yeast cells whose sole source of histone H3 was from a plasmid expressing these mutant proteins, and compared to that of an isogenic strain expressing the wild-type histone H3 protein from the same plasmid. Two biological replicates are included for each yeast strains expressing a specific histone H3 mutant. Three reference strains were also analyzed in the same way which expressed the wild-type histone protein. All strains were handled in the same way. A fresh culture of each strain was inoculated from a saturated overnight culture and grown to an OD A600 of approximately 0.7. Cells were harvested and total RNA extracted.

2010-05-27 | E-GEOD-13889 | biostudies-arrayexpress

Cells isolated from diaphragm of mdx mouse: satellite cells vs. PDGFRa+ cells

Project description:Transcriptional profiling of mouse skeletal muscle-derived cells comparing satellite cells with PDGFRa+ cells. Satellite cells and PDGFRa+ cells were directly isolated from diaphragm of dystrophic mdx mouse by FACS.

2011-12-08 | GSE25258 | GEO

Complementary patterns of gene expression by human oligodendrocyte progenitors and their environment predict determinants of progenitor maintenance and differentiation.

Project description:OBJECTIVE: Glial progenitor cells are abundant in adult human white matter. This study was designed to identify signaling pathways regulating their self-renewal and fate. METHODS: We compared the transcriptional profiles of freshly sorted adult human white matter progenitor cells (WMPCs), purified by A2B5-based immunomagnetic sorting, with those of the white matter from which they derived. RESULTS: We identified 132 genes differentially expressed by WMPCs; these included principal components of five receptor-defined signaling pathways, represented by platelet derived growth factor receptor alpha (PDGFRA) and type 3 fibroblast growth factor receptor (FGFR3), receptor tyrosine phosphatase-beta/zeta (RTPZ), notch, and syndecan3. WMPCs also differentially expressed the bone morphogenetic protein 4 (BMP4) inhibitors neuralin and BAMBI (BMP and activin membrane-bound inhibitor), suggesting tonic defense against BMP signaling. Differential overexpression of RTPZ was accompanied by that of its modulators pleiotrophin, NrCAM, tenascin, and the chondroitin sulfate proteoglycans, suggesting the importance of RTPZ signaling to WMPCs. When exposed to the RTPZ inhibitor bpV(phen), or lentiviral-shRNAi against RTPZ, WMPCs differentiated as oligodendrocytes. Conversely, when neuralin and BAMBI were antagonized by BMP4, astrocytic differentiation was induced, which was reversible by noggin. INTERPRETATION: The RTPZ and BMP pathways regulate the self-maintenance of adult human WMPCs, and can be modulated to induce their oligodendrocytic or astrocytic differentiation. As such, they provide targets by which to productively mobilize resident progenitor cells of the adult human brain. 6 samples, three biological replicates (individual patients), 2 groups (A2B5+ and unsorted cells).

2011-02-01 | E-GEOD-26535 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data