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Chromosomal High Affinity Binding Sites for the Drosophila Dosage Compensation Complex.

ABSTRACT: We identified 131 high affinity sites of the Drosophila DCC combining residual ChIP-chip profiles after differential crosslinking and RNAi-mediated knockdown of spreading factors Keywords: ChIP-chip MSL1 and MSL2 ChIP under various conditions including differential crosslinking and RNAi against MOF, MLE and MSL3. 2-4 replicates per experiment. dye-swaps as indicated in sample description.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Tobias Straub

PROVIDER: E-GEOD-12292 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The chromosomal high-affinity binding sites for the Drosophila dosage compensation complex.

Straub Tobias T Grimaud Charlotte C Gilfillan Gregor D GD Mitterweger Angelika A Becker Peter B PB

PLoS genetics 20081212 12

Dosage compensation in male Drosophila relies on the X chromosome-specific recruitment of a chromatin-modifying machinery, the dosage compensation complex (DCC). The principles that assure selective targeting of the DCC are unknown. According to a prevalent model, X chromosome targeting is initiated by recruitment of the DCC core components, MSL1 and MSL2, to a limited number of so-called "high-affinity sites" (HAS). Only very few such sites are known at the DNA sequence level, which has preclud ...[more]

PMID: 19079572

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